关键词: HO-8910细胞系, HO-8910PM细胞系, β-连环蛋白, 肝细胞生长因子, Transwell小室, 免疫印迹法, 人

Abstract: Objective To investigate associativity and mechanism of β-catenin with ovarian cancer metastasis. Methods The different expressions of β-catenin in high and low metastatic human ovarian serous cystadenocarcinoma cells（HO-8910PM and HO-8910） were detected by immunofluorescence staining, Western blotting and RT-PCR analysis. To further investigate the regulated mechanism of β-catenin with ovarian cancer metastasis, low metastatic human ovarian serous cystadenocarcinoma HO-8910 cells were treated by hepatocyte growth factor(HGF). The expression change of β-catenin in HO-8910 cells was investigated by the same technology mentioned above. Then, HO-8910 cells invasion and migration abilities were assessed by Transwell chambers and scratch wound migration assay. Results The expression of β-catenin was significantly lower in HO-8910PM cells than that in HO-8910 cells (P<0.05). HGF could downregulate the expression of β-catenin in HO-8910 cells (P<0.05) and enhance cell invasion (P<0.05) and migration. Conclusion The lower expression of β-catenin may be correlated with invasion and migration pot

Key words: HO-8910 cell line, HO-8910PM cell line, β-catenin, Hepatocyte growth factor, Transwell chamber, Western blotting, Human